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. 2014 Oct 30;13(18):2944–2951. doi: 10.4161/15384101.2014.947197

Figure 3.

Figure 3.

The ECT2 BRCT domain binds PAR during mitosis. (A) PAR is remarkably synthesized in vivo with the nocodazole treatment. (B) The in vivo interaction between PAR and ECT2 with or without the treatment of Tankyrase 1 siRNAs was measured by co-IP with the indicated antibodies. Whole cell lysates were blotted and shown as the input. (C) Tankyrase 1 protein was significantly decreased in Tankyrase 1 siRNA treated HeLa cells. β-actin was used as loading control. (D) The K519A mutant failed to interact with PAR in vivo. Wild type ECT2 was used as control. Whole cell lysates were blotted and shown as the input.