Figure 2.

Spliceosome depletion induces interphase delays. (A-B) HeLa cells transfected with the indicated siRNAs were synchronized in G1 by thymidine block and subsequently released into medium containing taxol. Samples were taken at the indicated time points after release and subjected to cell cycle profile analysis by flow cytometry using propidium iodide (PI) and anti-MPM2 staining. Overlaid DNA content histograms are shown. (C) Quantification of mitotic cells (MPM2+) for the experiment in (B). (D) Representative flow cytometry profiles of cells stained with PI and MPM2 at 12 h after release. The percentage of mitotic cells (MPM2+) is indicated. (E) Western blots of total lysates of cells in (B) blotted with the indicated antibodies. (F) Western blots of total lysates of cells in (B) at the time of release (t = 0 h) blotted with the indicated antibodies.