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. 2015 Oct 17;21:3136–3143. doi: 10.12659/MSM.895945

Figure 5.

Figure 5

Evaluation of NLRP3 inflammasome activation in Aldo-infused rats. (A) Relative expression of NLRP3 protein to β-actin expression. (B) Relative expression of ASC protein to β-actin expression. (C) Western blot analysis of NLRP3 and ASC. (D) Western blot analysis of mature IL-1β and mature IL-18. (E) mRNA expression of IL-1β normalized to GAPDH level. (F) mRNA expression of IL-18 normalized to GAPDH level. (G) Relative expression of mature IL-1β protein to β-actin expression. (H) Relative expression of mature IL-18 protein to β-actin expression. Data are presented as mean ±SEM; (n=6); * P<0.05, control versus Aldo-infused rats; # P<0.05, Aldo-infused rats versus Aldo + ROT treatment rats.