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. 2014 Oct 30;13(16):2554–2563. doi: 10.4161/15384101.2014.942204

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© 2014 The Author(s). Published with license by Taylor & Francis Group, LLC

© MiYoung Lee and Ashok R Venkitaraman

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 3. — Endogenous HMG20b localizes to the cytokinetic midbody. (A) Western blot analysis of control and HMG20b siRNA-treated HeLa cell extracts using HMG20b monoclonal antibody 1F6. Purified His-HMG20b protein was used as a positive control and β-Actin was probed as a loading control. (B) Confocal images of control and HMG20b siRNA-treated HeLa cells stained with anti-HMG20b monoclonal antibody 1F6 (green). MKLP1 (red) was co-stained as a midbody marker. Enlarged images of midbody are shown as insets. Scale bar is 5 μm. (C) Mean intensity of HMG20b staining at midbody. Results from 50 cells per sample are shown with mean ± s.e.m. (***: P < 0.001, unpaired t test)