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. 2015 Oct 21;5:15452. doi: 10.1038/srep15452

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(A) Negative-stain EM observation of the N-BAR mediated tubes. 20 μM of N-BAR was added to 720 μM 200 nm-extruded liposome and after 10 min incubation, embedded on a grid without dilution. (B) 0.2 μM of N-BAR was added to the same liposome as (A). Most of the vesicles remained intact with this condition. v- examples of intact vesicles t: examples of tubes. (C) Histogram of the distribution of the lengths of the tubes shown in (A). (D) Histogram of the distribution of the lengths of the tubes shown in (B).