Table 2. Identified protein spots that are differentially accumulated between hybrids of B. juncea (BJ) with transgenic B. napus (BJtBN) and of BJ with non-transgenic B. napus (BJBN).
| Spot ID | Accumulation Pattern | Ratio BJtBN/BJBN | Identified protein name | Accession number | Mascot score | Sequence coverage (%) | No. of sequenced/matched peptides | Exp. protein mass (kDa/pI) | Theor. protein mass (kDa/pI) | Function category | Function description |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 40 | H | 3.4 | myrosinase, thioglucoside glucohydrolase | CAA11412 | 1380 | 32 | 14/20 | 63/5.9 | 63/6.1 | metabolism | sugar & polysaccharide |
| 41 | H | 4.5 | myrosinase 2 | ADP24127 | 719 | 36 | 5/17 | 63/6.0 | 63/6.5 | metabolism | sugar & polysaccharide |
| 32 | H | 4.1 | BnaC01g09900D (cruciferin subunit) | CDY38381 | 581 | 17 | 7/9 | 93/7.8 | 53/7.6 | storage protein | storage protein |
| 43 | H | 1.9 | Cruciferin CRU4 | P33522 | 519 | 28 | 7/10 | 55/7.6 | 52/7.7 | storage protein | storage protein |
| 58 | H | 2.0 | PREDICTED: 12S seed storage protein CRD | XP_009118685 | 821 | 31 | 7/10 | 31/6.1 | 49/5.7 | storage protein | storage protein |
| 72 | H | A | cruciferin storage protein | BAJ78781 | 126 | 18 | 2/2 | 17/5.6 | 11/8.8 | storage protein | storage protein |
| 73 | H | 2.2 | cruciferin storage protein | BAJ78781 | 195 | 25 | 2/2 | 17/6.1 | 11/8.8 | storage protein | storage protein |
| 67 | H | A | BnaC03g55840D (17.4 kDa class I heat shock protein) | CDX86069 | 261 | 31 | 2/3 | 23/5.8 | 18/5.8 | cell defense & rescue | stress response |
| 70 | H | 1.9 | late embryogenesis-abundant protein | ABB55259 | 265 | 29 | 3/4 | 18/6.0 | 10/5.9 | cell defense & rescue | stress response |
| 39 | L | 0.4 | PREDICTED: beta-glucosidase 19 isoform X1 | XP_009109612 | 1060 | 38 | 10/16 | 69/6.7 | 61/6.4 | metabolism | sugar & polysaccharide |
| 53 | L | 0.6 | PREDICTED: provicilin | XP_009108832 | 145 | 3 | 1/1 | 38/6.6 | 55/6.6 | storage protein | storage protein |
| 34 | L | 0.4 | At2g42560 (late embryogenesis abundant domain-containing protein) | XP_002881860 | 119 | 4 | 1/2 | 90/6.6 | 67/6.0 | cell defense & rescue | stress response |
| 38 | L | 0.1 | At2g42560 (late embryogenesis abundant domain-containing protein) | XP_002881860 | 130 | 11 | 1/5 | 89/6.7 | 67/6.0 | cell defense & rescue | stress response |
| 61 | L | 0.4 | BnaA05g15190D (stress responsive A/B barrel domain protein) | CDY43909 | 364 | 33 | 3/5 | 28/4.9 | 23/4.9 | cell defense & rescue | stress response |
| 59 | L | 0.2 | PREDICTED: uncharacterized protein LOC103844119 | XP_009119147 | 144 | 18 | 3/3 | 30/6.1 | 27/5.8 | unknown | unknown |
Spot ID is the spot number shown in Fig. 3; accumulation pattern indicates the spot whose abundance is higher (H) or lower (L) in BJtBN seeds than BJBN seeds; Ratio BJtBN/BJBN, normalized spot volume in BJtBN seeds divided by the normalized volume in BJBN seeds; No. of sequence peptides/matched peptides, exp. protein mass and theo. protein mass: theoretical protein mass are described in Table 1. A: appeared. Proteins in the bracket are the homologous proteins of the identified proteins.