Table 3. Identified protein spots that are differentially accumulated between B. juncea (BJ) and hybrids of BJ with non-transgenic B. napus (BJBN).
| Spot ID | Accumulation Pattern | Ratio BJBN/BJ | Identified protein name | Accession number | Mascot score | Sequence coverage (%) | No. of sequenced/matched peptides | Exp. protein mass (kDa/pI) | Theor. protein mass (kDa/pI) | Function category | Function description |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 35 | H | 7.9 | cobalamin-independent methionine synthase | XP_002871787 | 1170 | 38 | 11/22 | 83/6.2 | 85/6.1 | metabolism | amino acid |
| 37 | H | 8.1 | hypothetical protein CARUB_v10000282 mg (cobalamin-independent methionine synthase) | XP_006286451 | 1380 | 39 | 13/20 | 84/6.3 | 85/6.2 | metabolism | amino acid |
| 39 | H | 3.1 | PREDICTED: beta-glucosidase 19 isoform X1 | XP_009109612 | 1060 | 38 | 10/16 | 69/6.7 | 61/6.4 | metabolism | sugar & polysaccharide |
| 41 | H | 1.8 | myrosinase 2 | ADP24127 | 719 | 36 | 5/17 | 63/6.0 | 63/6.5 | metabolism | sugar & polysaccharide |
| 42 | H | 2.5 | myrosinase | ABQ42337 | 1460 | 29 | 12/14 | 63/6.1 | 61/6.3 | metabolism | sugar & polysaccharide |
| 71 | H | 7.5 | BnaC03g70690D (nucleoside diphosphate kinase 1) | CDY10545 | 234 | 56 | 3/7 | 18/6.2 | 17/6.3 | metabolism | nucleotide |
| 51 | H | A | BnaCnng26110D (strictosidine synthase 1-like) | CDY54042 | 1060 | 52 | 9/16 | 48/6.4 | 42/6.1 | metabolism | others |
| 45 | H | 2.4 | ATPase alpha subunit | ABO86590 | 1080 | 29 | 9/14 | 57/6.2 | 55/6.0 | energy | respiration |
| 33 | H | 2.1 | PREDICTED: elongation factor 2-like | XP_009129918 | 1230 | 30 | 13/18 | 99/6.3 | 95/5.9 | protein synthesis | translational factor |
| 43 | H | 1.7 | Cruciferin CRU4 | P33522 | 519 | 28 | 7/10 | 55/7.6 | 52/7.7 | storage protein | storage protein |
| 46 | H | A | BnaC07g48660D (cruciferin BnC1) | CDY65916 | 590 | 33 | 6/10 | 55/6.7 | 51/7.2 | storage protein | storage protein |
| 53 | H | A | PREDICTED: provicilin | XP_009108832 | 145 | 3 | 1/1 | 38/6.6 | 55/6.6 | storage protein | storage protein |
| 65 | H | 6.0 | cruciferin cru2/3 subunit | CAA40979 | 300 | 73 | 3/5 | 22/6.0 | 54/6.8 | storage protein | storage protein |
| 66 | H | 4.4 | cruciferin cru2/3 subunit | CAA40979 | 617 | 57 | 4/5 | 22/6.6 | 54/6.8 | storage protein | storage protein |
| 68 | H | 1.5 | hypothetical protein CARUB_v10012738 mg (12S seed storage protein ) | XP_006279075 | 437 | 12 | 5/5 | 22/6.1 | 52/6.7 | storage protein | storage protein |
| 73 | H | 2.2 | cruciferin storage protein | BAJ78781 | 195 | 25 | 2/2 | 17/6.1 | 11/8.8 | storage protein | storage protein |
| 36 | H | 4.2 | PREDICTED: embryonic protein DC-8 | XP_009142073 | 727 | 16 | 6/8 | 84/6.1 | 73/6.0 | cell structure | others |
| 44 | H | 2.6 | PREDICTED: myrosinase-binding protein-like At3g16440 | XP_009109600 | 923 | 47 | 8/24 | 57/6.2 | 50/6.1 | cell defense & rescue | defense-related |
| 55 | H | A | BnaC05g11200D (myrosinase-binding protein-like) | CDY53520 | 920 | 49 | 8/14 | 35/6.0 | 31/5.9 | cell defense & rescue | defense-related |
| 38 | H | 12.6 | At2g42560 (late embryogenesis abundant domain-containing protein) | XP_002881860 | 130 | 11 | 1/5 | 89/6.7 | 67/6.0 | cell defense & rescue | stress response |
| 49 | H | A | PREDICTED: cytosolic isocitrate dehydrogenase [NADP] | XP_009127460 | 1480 | 73 | 14/32 | 52/6.4 | 46/6.3 | cell defense & rescue | stress response |
| 70 | H | 2.0 | late embryogenesis-abundant protein | ABB55259 | 265 | 29 | 3/4 | 18/6.0 | 10/5.9 | cell defense & rescue | stress response |
| 48 | H | 2.6 | PREDICTED: alcohol dehydrogenase class-3 | XP_009101752 | 996 | 45 | 9/10 | 52/6.7 | 42/6.5 | cell defense & rescue | detoxification |
| 57 | H | 1.7 | peroxiredoxin antioxidant | AAF61460 | 690 | 74 | 6/9 | 31/6.2 | 24/6.0 | cell defense & rescue | detoxification |
| 47 | L | 0.2 | PREDICTED: cruciferin CRU4 | XP_009119353 | 380 | 19 | 5/8 | 53/6.8 | 52/6.8 | storage protein | storage protein |
| 62 | L | 0.5 | cupin domain-containing protein | NP_180416 | 248 | 9 | 3/4 | 25/5.1 | 56/5.8 | storage protein | storage protein |
| 64 | L | 0.3 | BnaA07g13950D (vicilin-like antimicrobial peptides 2-2) | CDY04569 | 222 | 11 | 2/3 | 23/6.0 | 52/6.0 | cell defense & rescue | defense-related |
| 34 | L | 0.3 | At2g42560 (late embryogenesis abundant domain-containing protein) | XP_002881860 | 119 | 4 | 1/2 | 90/6.6 | 67/6.0 | cell defense & rescue | stress response |
| 54 | L | 0.3 | PREDICTED: late embryogenesis abundant protein D-34-like | XP_009145325 | 140 | 42 | 1/6 | 36/4.7 | 27/4.7 | cell defense & rescue | stress response |
| 61 | L | 0.5 | BnaA05g15190D (stress responsive A/B barrel domain protein) | CDY43909 | 364 | 33 | 3/5 | 28/4.9 | 23/4.9 | cell defense & rescue | stress response |
| 69 | L | 0.4 | BnaC09g44630D (17.6 kDa class II heat shock protein) | CDX97162 | 288 | 34 | 2/3 | 21/6.2 | 17/6.2 | cell defense & rescue | stress response |
| 50 | L | 0.5 | PREDICTED: alcohol dehydrogenase class-3 | XP_009101752 | 854 | 45 | 9/12 | 52/6.6 | 42/6.5 | cell defense & rescue | detoxification |
| 60 | L | 0.2 | Fe superoxide dismutase 1, partial | ADR01109 | 194 | 12 | 2/3 | 42/8.1 | 22/5.8 | cell defense & rescue | detoxification |
| 59 | L | 0.4 | PREDICTED: uncharacterized protein LOC103844119 | XP_009119147 | 144 | 18 | 3/3 | 30/6.1 | 27/5.8 | unknown | unknown |
Spot ID is the spot number shown in Fig. 4; accumulation pattern indicates the spot whose abundance is higher (H) or lower (L) in BJBN seeds than BJ seeds; Ratio BJBN/BJ, normalized spot volume in BJBN seeds divided by the normalized volume in BJ seeds; No. of sequence peptides/matched peptides, exp. protein mass and theo. protein mass: theoretical protein mass are described in Table 1. A: appeared. Proteins in the bracket are the homologous proteins of the identified proteins.