Figure 1.
PML suppressed lung cancer cell invasion when EGFR was activated. (A) Left, Luciferase-expressing A549-shControl or shPML cells were injected via the tail vein into immunocompromised NOD/SCID mice (n = 5 for each group). The mice were monitored for colonization of the injected cells in the lungs as described in the Materials and Methods. Representative images from week 6 post-injection are shown. Right, The mean total photon flux measured by IVIS at week 6 is shown. The data are presented as the mean ± SEM. (B) The cumulative survival rate of the same mice described in Figure 1A was monitored weekly until 13th week after cell injection. (C) Left, H1975-shControl and H1975-shPML cell invasion was assayed as described in the Materials and Methods. Right, A549-shControl and A549-shPML cells were cultured overnight in low-serum starvation conditions (0.5% FBS). These cells were then subjected to the cell invasion assay in the low serum-medium with 50 ng/ml EGF. (D) A549-Control and A549-PML cells were cultured overnight in low-serum starvation conditions (0.5% FBS). These cells were then subjected to the cell invasion assay in the low serum-medium with 50 ng/ml EGF. (E) CL1-5-Control and CL1-5-PML cell invasion was assayed as described in (D).