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. 2014 Oct 30;13(19):3132–3142. doi: 10.4161/15384101.2014.949212

Figure 5.

Figure 5.

PML repressed the coactivation of the MMP2 promoter by nEGFR and STAT3. (A) 293T cells transfected with pMMP2 Luc, pCMV-NLS-EGFR, the STAT3 expression vector and increasing amounts of the PML expression plasmid were subjected to a luciferase reporter assay. (B) PML-knockdown H1975 cells with or without shRNA-resistant PML expression were subjected to ChIP with preimmune IgG or an anti-EGFR or anti-STAT3 antibody. The relative amount of the immunoprecipitated MMP2 ATRS was quantified by RT-qPCR. (C and D) PML-knockdown H1975 cells with or without shRNA-resistant PML expression were subjected to ChIP with preimmune IgG or an anti-PML or anti-H3K9Ac antibody. The relative amount of the immunoprecipitated MMP2 ATRS was quantified by RT-qPCR. (E) Left, H1975 cells infected with control, shEGFR or shSTAT3-2 lentiviral vectors were subjected to Western blotting with indicated antibodies 2 d after infection. Right, The same stable cell lines as in the left panel were subjected to ChIP with preimmune IgG or an anti-PML antibody. The amount of immunoprecipitated MMP2 ATRS was quantified by RT-qPCR.