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. 2014 Oct 30;13(19):3132–3142. doi: 10.4161/15384101.2014.949212

Figure 6.

Figure 6.

PML upregulation was involved in the IFNβ-mediated suppression of lung cancer cell invasion. (A) After overnight serum starvation, A549 cells were treated with EGF and IFNβ for the indicated periods of time. The cells were then subjected to RT-qPCR and Western blotting for PML mRNA and protein expression, respectively. (B) After overnight serum starvation, H1975 cells were treated with IFNβ for the indicated periods of time. The cells were analyzed as in (A). (C) After overnight serum starvation, A549 cells were treated with EGF and IFNβ for the indicated periods of time. The relative MMP2 mRNA expression was quantified by RT-qPCR with specific primers. (D) Control and PML-knockdown A549 cells were treated with EGF or IFNβ for 3 d and then analyzed by RT-qPCR to determine the relative MMP2 mRNA expression. (E) A549 cells treated with EGF and/or IFNβ for 24 h were subjected to a cell invasion assay as described in the Materials and Methods.