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. 1988 Sep;43(9):669–673. doi: 10.1136/thx.43.9.669

Binding and diffusion characteristics of 14C EDTA and 99mTc DTPA in respiratory tract mucus glycoprotein from patients with chronic bronchitis.

M S Cheema 1, S Groth 1, C Marriott 1
PMCID: PMC461453  PMID: 3143162

Abstract

Measurement of pulmonary clearance of an inhaled aerosol of technetium-99m labelled diethylenetriaminepenta-acetate (DTPA) by external detection methods has been used widely as an index of permeability across alveolar epithelium and bronchial mucosa. To determine the applicability of the tracer to measurement of permeability in the airways the diffusion and binding characteristics of 99mTc DTPA and the chemically related ethylenediaminetetra-acetic acid labelled with carbon-14 (14C EDTA) was studied in purified respiratory tract mucus glycoprotein from patients with chronic bronchitis. The diffusion coefficients for 99mTc DTPA and 14C EDTA through mucus gels were significantly lower than those for tritiated water. Both molecules bound to the mucus gels with high affinity at two independent low capacity sites. Appreciable amounts of 99mTc DTPA or 14C EDTA are therefore unlikely to cross mucus layers of physiological thickness over periods of four or five hours. This suggests that when pulmonary clearance is determined by the 99mTc DTPA method the tracer retained in mucus lined airways will provide background activity. This study supports the assumption that pulmonary clearance measurements are mainly measuring alveolar epithelial permeability and should not be used to study bronchial epithelial permeability.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chaudhuri T. K., Greenwald A. J., Heading R. C. Measurement of gastric emptying time--a comparative study between nonisotopic aspiration method and new radioisotopic technique. Am J Dig Dis. 1975 Nov;20(11):1063–1066. doi: 10.1007/BF01071195. [DOI] [PubMed] [Google Scholar]
  2. Chaudhuri T. K. Use of 99mTc-DTPA for measuring gastric emptying time. J Nucl Med. 1974 Jun;15(6):391–395. [PubMed] [Google Scholar]
  3. Christian P. E., Moore J. G., Sorenson J. A., Coleman R. E., Weich D. M. Effects of meal size and correction technique on gastric emptying time: studies with two tracers and opposed detectors. J Nucl Med. 1980 Sep;21(9):883–885. [PubMed] [Google Scholar]
  4. Delin N. A., Axelsson B., Johansson C., Poppen B. Comparison of gamma camera and withdrawal methods for the measurement of gastric emptying. Scand J Gastroenterol. 1978;13(7):867–872. doi: 10.3109/00365527809182205. [DOI] [PubMed] [Google Scholar]
  5. Dusser D. J., Collignon M. A., Stanislas-Leguern G., Barritault L. G., Chrétien J., Huchon G. J. Respiratory clearance of 99mTc-DTPA and pulmonary involvement in sarcoidosis. Am Rev Respir Dis. 1986 Sep;134(3):493–497. doi: 10.1164/arrd.1986.134.3.493. [DOI] [PubMed] [Google Scholar]
  6. Elwood R. K., Kennedy S., Belzberg A., Hogg J. C., Paré P. D. Respiratory mucosal permeability in asthma. Am Rev Respir Dis. 1983 Sep;128(3):523–527. doi: 10.1164/arrd.1983.128.3.523. [DOI] [PubMed] [Google Scholar]
  7. Jacobs M. P., Baughman R. P., Hughes J., Fernandez-Ulloa M. Radioaerosol lung clearance in patients with active pulmonary sarcoidosis. Am Rev Respir Dis. 1985 May;131(5):687–689. doi: 10.1164/arrd.1985.131.5.687. [DOI] [PubMed] [Google Scholar]
  8. Jones J. G., Minty B. D., Lawler P., Hulands G., Crawley J. C., Veall N. Increased alveolar epithelial permeability in cigarette smokers. Lancet. 1980 Jan 12;1(8159):66–68. doi: 10.1016/s0140-6736(80)90493-6. [DOI] [PubMed] [Google Scholar]
  9. Kennedy S. M., Elwood R. K., Wiggs B. J., Paré P. D., Hogg J. C. Increased airway mucosal permeability of smokers. Relationship to airway reactivity. Am Rev Respir Dis. 1984 Jan;129(1):143–148. doi: 10.1164/arrd.1984.129.1.143. [DOI] [PubMed] [Google Scholar]
  10. Lifschitz M. I., Denning C. R. Quantitative interaction of water and cystic fibrosis sputum. Am Rev Respir Dis. 1970 Sep;102(3):456–458. doi: 10.1164/arrd.1970.102.3.456. [DOI] [PubMed] [Google Scholar]
  11. Mason G. R., Uszler J. M., Effros R. M., Reid E. Rapidly reversible alterations of pulmonary epithelial permeability induced by smoking. Chest. 1983 Jan;83(1):6–11. doi: 10.1378/chest.83.1.6. [DOI] [PubMed] [Google Scholar]
  12. Oberdörster G., Utell M. J., Morrow P. E., Hyde R. W., Weber D. A. Bronchial and alveolar absorption of inhaled 99mTc-DTPA. Am Rev Respir Dis. 1986 Nov;134(5):944–950. doi: 10.1164/arrd.1986.134.5.944. [DOI] [PubMed] [Google Scholar]
  13. Rinderknecht J., Shapiro L., Krauthammer M., Taplin G., Wasserman K., Uszler J. M., Effros R. M. Accelerated clearance of small solutes from the lungs in interstitial lung disease. Am Rev Respir Dis. 1980 Jan;121(1):105–117. doi: 10.1164/arrd.1980.121.1.105. [DOI] [PubMed] [Google Scholar]

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