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. 2015 Jan 7;14(6):808–819. doi: 10.1080/15384101.2014.1000685

Figure 4.

Figure 4.

For figure legend, see page 816.

Figure 4.

Figure 4.

For figure legend, see page 816.

Figure 4.

Figure 4.

FUCCI-expressing cancer cells on Gelfoam® have the same cell cycle response to cytotoxic agents as subcutaneous tumors. Time-course imaging of FUCCI-expressing cancer cells (A) subcutaneous, (B) in monolayer culture, (C) in tumor spheres on agar and (D) on Gelfoam®, before and after chemotherapy. (E) Representative images of FUCCI-expressing cancer cells in monolayer culture, spheres on agar, on Gelfoam®, and subcutaneous tumors, before and after chemotherapy with cisplatinum or paxlitaxel. In monolayer culture, chemotherapy blocked cancer cells in S/G2/M phase. Chemotherapy had little effect on quiescent tumor spheres. In contrast, tumors on Gelfoam® histoculture and subcutaneous tumors had a similar initial response to chemotherapy with cells becoming blocked in G0/G1. (F) Histograms of cell-cycle phase distribution before and after chemotherapy of 2D monolayer, sphere and Gelfoam® cultures and subcutaneous tumors. (Also see Video S1).