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. 2015 May 6;14(14):2274–2284. doi: 10.1080/15384101.2015.1046654

Table 1.

List of primers

Gene Symbol Forward Primer (5′-3′) Reverse Primer (5′-3′)
CRT TGGAAGACGATTGGGACTTC TCCTCAGGCTTGGAGTCTGT
p53 TTGCAATAGGTGTGCGTCAGA AGTGCAGGCCAACTTGTTCAG
MAD2L1 CCTAAATAATGTGGTGGAACAACTG CTGTCATCTTTTGCAGTCTTGTC
CBFB AGGCACGCTTCCAGAAC TTTGTCGCTGTTCTCCCTG
YWHAG ACTGGTGCAGAAAGCCC ACGTTCTTGTAGGCCACAG
PAH TTGGATAAACGTAGCCTGCC AGCTCTTGAATGGTTCTTGGG
SNRPB GAAATCGGCTGTGGGAGAG TGCACCTCATCCTGTAATCAAT
EIF4EBP2 CAATATCCCAGGAGTCACTAGC CTTGCAGGAGAGTCAGATGTC
WBP1 GAGTGAACAACCAGCCCTAC CAGCTAAAGAGGATGAGGACAG
SHBG AGGATGACTGGTTTATGCTGG CCCTCCATCTTGACTTCCAC
MYBPHL CATTGACATCCTGGTGATTGAG AAGTGCTGTATTCCCCGTATC
ITIH1 CAGATGGAAACGCATTTATCGG GGTGAGGTGGATGGTGAATTG
ChIP_p53_Site 1
aatgaagtttgggatgggcc
Tcattttatccagcctcgcg
Cloning Primers
Forward Primer (5′-3′)
Reverse Primer (5′-3′)
SREBP-1c_ promoter TGCGAGCTCGGCCCACTGGTATCCTATCC GGAAGATCTCTCGTACGGCCCTTCCTA
p53_cDNA_clone
CGCGGATCCACTGCCTTCCGGGTCACT
CCGCTCGAGGACCCAAAACCCAAAATGG
Mutations Primers
Sense (5′-3′)
Antisense (5′-3′)
SREBP-1c promoter_ Site1_MUT GCCCAGAGAGGGGGCTCGAAGCTTCCGCGTTCCCGCAGCAGC GCTGCTGCGGGAACGCGGAAGCTTCGAGCCCCCTCTCTGGGC
SREBP-1c promoter_ Site2_MUT
GGGGCAGGCTGATCTCACAGGTTCTAGACCCCTCCACATTGTCACT
AGTGACAATGTGGAGGGGTCTAGAACCTGTGAGATCAGCCTGCCCC
EMSA Primers
Sense (5′-3′)
Antisense (5′-3′)
EMSA_Site 1 ggctcggcttgcccgcgt Acgcgggcaagccgagcc