CRT |
TGGAAGACGATTGGGACTTC |
TCCTCAGGCTTGGAGTCTGT |
p53 |
TTGCAATAGGTGTGCGTCAGA |
AGTGCAGGCCAACTTGTTCAG |
MAD2L1 |
CCTAAATAATGTGGTGGAACAACTG |
CTGTCATCTTTTGCAGTCTTGTC |
CBFB |
AGGCACGCTTCCAGAAC |
TTTGTCGCTGTTCTCCCTG |
YWHAG |
ACTGGTGCAGAAAGCCC |
ACGTTCTTGTAGGCCACAG |
PAH |
TTGGATAAACGTAGCCTGCC |
AGCTCTTGAATGGTTCTTGGG |
SNRPB |
GAAATCGGCTGTGGGAGAG |
TGCACCTCATCCTGTAATCAAT |
EIF4EBP2 |
CAATATCCCAGGAGTCACTAGC |
CTTGCAGGAGAGTCAGATGTC |
WBP1 |
GAGTGAACAACCAGCCCTAC |
CAGCTAAAGAGGATGAGGACAG |
SHBG |
AGGATGACTGGTTTATGCTGG |
CCCTCCATCTTGACTTCCAC |
MYBPHL |
CATTGACATCCTGGTGATTGAG |
AAGTGCTGTATTCCCCGTATC |
ITIH1 |
CAGATGGAAACGCATTTATCGG |
GGTGAGGTGGATGGTGAATTG |
ChIP_p53_Site 1
|
aatgaagtttgggatgggcc
|
Tcattttatccagcctcgcg
|
Cloning Primers
|
Forward Primer (5′-3′)
|
Reverse Primer (5′-3′)
|
SREBP-1c_ promoter |
TGCGAGCTCGGCCCACTGGTATCCTATCC |
GGAAGATCTCTCGTACGGCCCTTCCTA |
p53_cDNA_clone
|
CGCGGATCCACTGCCTTCCGGGTCACT
|
CCGCTCGAGGACCCAAAACCCAAAATGG
|
Mutations Primers
|
Sense (5′-3′)
|
Antisense (5′-3′)
|
SREBP-1c promoter_ Site1_MUT |
GCCCAGAGAGGGGGCTCGAAGCTTCCGCGTTCCCGCAGCAGC |
GCTGCTGCGGGAACGCGGAAGCTTCGAGCCCCCTCTCTGGGC |
SREBP-1c promoter_ Site2_MUT
|
GGGGCAGGCTGATCTCACAGGTTCTAGACCCCTCCACATTGTCACT
|
AGTGACAATGTGGAGGGGTCTAGAACCTGTGAGATCAGCCTGCCCC
|
EMSA Primers
|
Sense (5′-3′)
|
Antisense (5′-3′)
|
EMSA_Site 1 |
ggctcggcttgcccgcgt |
Acgcgggcaagccgagcc |