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. 2015 Jun 23;14(16):2578–2589. doi: 10.1080/15384101.2015.1033591

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Figure 6. — Effect of amantadine, ATRA, or the amantadine-ATRA combination on PU.1 and VDR expression in HL60 cells. Levels of PU.1 and VDR mRNA (A, B) or protein (C, D) detected by real time PCR and western blotting, respectively, in untreated or drug-treated HL-60 cells; (E, F) Densitometric analysis from 2 combined experiments. Expression of Grb2 was detected as loading control. Statistical significance was calculated using unpaired, 2-tailed Student's t test and considered significant with p < 00.05. “NS” indicates not statistically significant differences.