Figure 3.

The H3 K27 acetylation-enriched CNS10 is a strong enhancer element for the Pparg2 gene. (A) A schematic of the constructed firefly luciferase reporter plasmid. The 0.6 kb Pparg2 promoter was amplified and inserted upstream of the firefly luciferase gene in the pGL3 vector. The 11 CNS were inserted individually at the 3′-end of the luciferase gene. (B) The firefly luciferase reporter vectors containing no CNS (negative control) or CNS1 - 11 were transfected into C3H 10T1/2 cells along with a renilla luciferase vector (pRLTK) as an internal control. The activities of both the firefly and renilla luciferases were measured 48 hours after transfection. These results are the averages of 3 to 6 independent luciferase assays, and the error bars represent standard deviations.