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. 2015 Jan 7;14(7):1090–1102. doi: 10.1080/15384101.2014.1000695

Figure 7.

Figure 7.

SOCS3 and STAT3 expression and effects of SOCS3 silencing in TAX and TAX+TNF-treated HCC cells. (A) Representative protein gel blots and (B) densitometric quantification of SOCS3, STAT3 and pSTAT3 levels in Huh7 and HepG2 cells after a 24 hour treatment with 0.1 μM TAX or 15 ng/ml TNF or TAX+TNF. Data are means±SD expressed as percentage of controls. Significance of the differences vs controls: **P < 0.01 and ***P < 0.001; vs TAX: °°°P < 0.001. (C) Representative western blot pattern of SOCS3 levels in HepG2 and Huh7 cells untreated or treated with siSOCS3 (100 pmol) for 24 and 48 hours. (D) Effect of SOCS3-siRNA or siC (100 pmol) on TNF-induced apoptosis: cells were transfected with siSOCS3 or nontargeting siRNA (siC) for 48 hours, then exposed to TNF (15 ng/ml) for further 24 hours. Histograms represent percentages of hypodiploid (apoptotic) cells in the culture. Data are means±SD of 4 experiments. Significance of the differences: vs controls **P < 0.01 and ***P < 0.001; vs TNF and siSOCS3: §§§P <0.001.