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. 2015 Jan 3;13(24):3857–3866. doi: 10.4161/15384101.2014.972875

Figure 2.

Figure 2.

Inhibition of Notch signaling in esophageal epithelial cells changes basal cell morphology in 3D cultures. (A) Western blotting for GFP (dnMAML), MYC and CDX1 in EPC2-hTERT, MYC-CDX1 and MYC-CDX1-dnMAML cells. (B) Luciferase assay with Notch-responsive pGL3–8XCSL reporter vector in MYC-CDX1-ICN1 and MYC-CDX1-ICN1-dnMAML cells, graph represents mean ± SEM (n = 3). Student t-test was performed to determine significance, *P ≤ 0.05. (C) Quantitative PCR (qPCR) for Notch downstream targets HES1 and HES5 in MYC-CDX1 and MYC-CDX1-dnMAML cells. Graph represents mean ± SEM (n = 3) and student t-test was performed to determine significance, *P ≤ 0.05. (D) H&E staining of representative 3D organotypic cultures of MYC-CDX1 and MYC-CDX1-dnMAML cells, arrow indicates elongated cells, (200X Magnification). (E) Electron microscopy of MYC-CDX1 and MYC-CDX1-dnMAML 3D organotypic cultures, scale bars = 0.2 μm. (F) Graph represents relative height of MYC-CDX1 and MYC-CDX1-dnMAML basal layer cells mean ± SEM (n = 4). Student t-test was performed to determine significance, *P ≤ 0.0001.