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. 2015 Jan 3;13(24):3857–3866. doi: 10.4161/15384101.2014.972875

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Figure 5. — KLF4 knockdown reverses partially the morphological changes induced by Notch signaling inhibition in 3D cultures. (A) qPCR of KLF4 expression in MYC-CDX1 and MYC-CDX1-dnMAML cells. (B) Western blotting for KLF4 in EPC2-hTERT, MYC-CDX1 and MYC-CDX1-dnMAML cells. (C) qPCR of KLF4 in MYC-CDX1-dnMAML-shScramble and MYC-CDX1-dnMAML-shKLF4 cells. (D) Western blotting for KLF4 in MYC-CDX1-dnMAML-shScramble and MYC-CDX1-dnMAML-shKLF4 cells. (E) H&E staining of MYC-CDX1-dnMAML-shScramble and MYC-CDX1-dnMAML-shKLF4 3D organotypic cultures (400X Magnification). Graph represents mean ± SEM (n = 6). Student t-test was performed to determine significance, *P ≤ 0.01.