KLF4 knockdown reverses lineage changes induced by Notch signaling inhibition in esophageal epithelial cells. qPCR of (A) columnar keratins K8, K18, K19 and K20; (B) mucin genes MUC2, MUC3B, MUC5B and MUC17; (C) squamous keratins K5, K13 and K14; (D) and squamous differentiation markers DSC1 and DSC3 in MYC-CDX1-dnMAML-shScramble and MYC-CDX1-dnMAML-shKLF4 cells. Graph represents mean ± SEM (n = 6). Student t-test was performed to determine significance, *P ≤ 0.01, **P ≤ 0.001.