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CLN1 but not CLN2 is required for G1 delay induced by cAMP addition. Asynchronous cultures of the GG104 cln1Δ (A) and GG104 cln2Δ (B) strains growing exponentially (1.7–2 × 106 cells/ml) in YPD medium were splitted into 2 fractions and 2mM cAMP was added to one of them (▪), while the other was the untreated control (♦). Samples were harvested every 30 minutes to assay cell number, cell size and budding index.
