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GG104 swi4Δ mutant does not show G1/S transient arrest after PKA activation. Asynchronous cultures of the GG104 swi4Δ growing in YPD medium, GG104 swi4Δ [YCplac111-SWI4S159A], GG104 swi4Δ [YCplac111-SWI4] strains growing exponentially in selective medium were splitted into 2 fractions and 2mM cAMP was added to one of them (▪), while the other was the untreated control (Δ). Samples were harvested every 30 minutes to assay cell number, cell size and budding index.
