Table 1.
Summary of the MAGE-RING analysis. Most of the RING-finger proteins bind through their diverse C-termini to primarily WH/B subdomains of their MAGE partners. Significant contribution of the WH/A subdomain was visible only for MAGEG1-NSE1 and MAGEA1-TRIM31. Stimulation of ubiquitin ligase activity was observed only for MAGEG1-NSE1 and MAGEA1-TRIM31.
| binding domain | MHD subdomain | Stimulation of ubiquitination | |
|---|---|---|---|
| NSE1 | WH | WH/A | Yes |
| MDM4 | ND | WH/B | No |
| PCGF6 | C-term. | WH/B>WH/A | No |
| RNF166 | C-term. | WH/B>>WH/A | No |
| TRAF6 | C-term. | ND | ND |
| TRIM8 | C-term. | WH/B>WH/A | No |
| TRIM31 | coiled-coil | WH/A∼WH/B | Yes |
| TRIM41 | C-term. | ND | No |
Summary of binding domain analysis of the RING-finger proteins is based on Fig. 2C and Fig. S4. WH/A and WH/B (MHD subdomain) data are from Fig. 2A and Fig. S3. Ubiquitination of RING and MAGE proteins was followed during co-immunoprecipitation (Fig. 1, Figs. S1 and S2) and ubiquitination (Fig. 4 and Fig. S5) experiments. Specifically, data for MAGEG1-NSE1 are from Fig. S3 and Doyle et al.12 ND – not determined.