Figure 4. slr0815 gene deletion mutation and its effect on NDH-CET.

(a) Construction of plasmid used to generate the slr0815 deletion mutant (∆slr0815). (b) PCR segregation analysis of the ∆slr0815 mutant using the slr0815-G and slr0815-H primer sequences (Supplemental Table S4). See Supplementary Figure S3 for full views of DNA electrophoresis gels. (c) Monitoring of NDH-CET activity by chlorophyll fluorescence. Two-day-old cells were exposed to actinic light (45 μmol photons m⁻²s⁻¹) for 30 sec. After illumination, the subsequent transient change in chlorophyll fluorescence was monitored as an indication of NDH-CET activity. a.u., arbitrary units.