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The effect of cytotoxic drugs on WT1 and apoptosis in MCF7 cells. Cells were treated with 1 μg/ml or 5 μg/ml of each drug for 20 hours and whole cell extracts prepared, then immunoblotted with the antibodies indicated. To measure cell viability, cells were treated as above, stained with trypan blue and the stained/excluded cells counted and expressed graphically as percentage viable.
