Figure 1.
Generation of TIF-IAΔ/Δ mouse model to study β-cell regeneration. (A) β-cell specific deletion of TIF-IA is used to induce apoptosis. YFP labeling marks the recombined cells. (B) Apoptotic β-cells are detected in the islet of TIF-IAΔ/Δ mice. (C) Representative immunostaining of insulin (red), glucagon (gray), and YFP (green) in the pancreata of TIF-IA+/+ and TIF-IAΔ/Δ mice at different time points after tamoxifen injection. (D) Quantification of insulin and YFP expressing cells shows 95% YFP-labeling upon Cre-mediated recombination. In TIF-IAΔ/Δ mutants, YFP-labeled cells are lost gradually, and replaced with non-labeled cells (D). mo p.inj.: months post injection. Scale bar: 25 μm. Values are presented by mean ± SEM. N >3 animals for each experimental condition.