Figure 2.

β-cell ablation and regeneration in TIF-IA^Δ/Δ mice. (A) Massive β-cell ablation leads to development of severe and long-lasting hyperglycemia in TIF-IA^Δ/Δ mice. Mutant mice gradually recover from hyperglycemia. (B and C) Immunostaining and quantification of insulin (green) and glucagon (red) expressing cells in the pancreata of TIF-IA^+/+ and TIF-IA^Δ/Δ mice at different time points post injection (A). β-cell number is increased gradually in mice injected at one-month (B). (D) Measurement of blood insulin level confirms the changes in functional β-cell number. mo p.inj.: months post injection. Scale bar: 25 μm. Values are presented by mean ± SEM. P-value <0.05 : *, P-value <0.01: **. N >3 animals for each experimental condition.