Figure 3.

Ectopically expressed human SYCP2L localizes to centromeres in 293T cells. (A) Properties of five human SYCPL2 proteins resulting from SNPs. Three SNPs result in a missense mutation, and two SNPs cause a termination codon leading to C-terminal truncation. There are only three missense SNPs with a minor allele frequency (MAF) of >0.01 in human SYCP2L gene. Corresponding minor allele residues or termination codons are shown in red. (B) Western blot analysis of human SYCP2L mutants in transfected 293T cells. V5-SYCP2L protein variants were detected using an anti-V5 tag antibody; ACTB served as a loading control. (C) Localization of wild-type and mutant human SYCP2L variants in transfected 293T cells. V5-SYCP2L protein and centromeres were identified by immunostaining with an anti-V5 antibody and CREST antiserum, respectively (also shown in separate channels). DNA was stained with DAPI. Scale bar, 20 µm.