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. 2015 Jan 15;14(4):566–576. doi: 10.4161/15384101.2014.991166

Figure 1.

Figure 1.

RS inhibits NF-κB in an ATR- and p50-dependent manner. (A) Human ATM−/− and ATM+/+ cells were treated with Hu (2 mM) and EMSA performed after isolation of nuclear extracts. Competition was performed with specific (SC) and non-specific (NS) cold DNA. (B) NF-κB EMSA (upper) and immunoblot (IB, lower) using nuclear extract from ATM−/− cells transfected with the indicated siRNA and treated with Hu (2 mM, 4 hrs). (C) IB in nuclear and cytoplasmic fractions from ATM−/− cells treated with Hu (2 mM) (p-p50: anti-phospho-S329-p50 antibody). (D) NF-κB-dependent luciferase assay in ATM−/− cells transfected with siRNA and treated with vehicle or 2 mM Hu (4 hrs). Data show mean value relative to renilla, normalized to control, ± SD of triplicate samples. *P < 0.05 relative to untreated.