Skip to main content
. 2015 Jan 15;14(4):566–576. doi: 10.4161/15384101.2014.991166

Figure 3.

Figure 3.

ATR inhibits NF-κB activity independent of DNA damage. (A) 293T cells stably expressing TopBP1ER were transfected with the indicated siRNA and stimulated with TAM (500 nM). IB and EMSA (NF-κB and Oct1) were performed on nuclear extracts isolated at the indicated time. p-ATR (anti-phospho-Ser428 ATR). (B) NF-κB-dependent luciferase assay in 293T-TopBP1ER cells expressing the indicated siRNA following stimulation with TAM (8 hrs). *P < 0.01. (C) Nfkb1−/− MEFs stably expressing p50WT or p50S329A were infected with TopBP1ER and stimulated with TAM (500 nM). EMSA was performed on nuclear extracts using the indicated probe. IB shows equal p50 expression in cells. (D) qPCR of Bclxl and Bax mRNA expression in cells from (C) following stimulation with TAM (8 hrs). Data show mean normalized value, ± SEM of triplicate samples from 3 experiments. *P < 0.05 relative to untreated.