Figure 6.
p50 S329 is necessary to maintain genome stability. (A–C) Primary Nfkb1−/−MEFs were infected at passage 1 with the indicated p50 construct and analyses made after serial passage. Experiments were performed using at least 3 separate MEF isolations/ infections expressing equivalent levels of p50. (A) γH2AX foci. *P < 0.001 relative to p50WT-expressing cells. Inset: IB with anti-p50. Data from (A and B) show mean ± SD (n ≥ 150 cells). (B) β-gal staining. *P < 0.005. (C) Spontaneous breaks and gaps. n = 25 metaphase spreads per sample. Data show mean ± SEM. *P < 0.005 relative to p50WT-expressing cells. Representative metaphase spreads shown (right). Arrows indicate chromosomal gaps/breaks. P6 cells expressing p50S329A have multiple/severe breaks.