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. 2015 Jan 15;14(6):902–919. doi: 10.1080/15384101.2014.1000197

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Figure 5. — Nucleolin belongs to protein complexes containing centrosomal proteins. (A) γ-tubulin coimmunoprecipitates with nucleolin. Immunoprecipitation (IP) assay was performed in HeLa cells with anti nucleolin polyclonal antibody (polyclonal antibody 5567). Following immunoprecipitation, proteins were separated on a SDS-PAGE gel and probed with an anti nucleolin polyclonal antibody (polyclonal antibody 134, first row), detected with a secondary antibody coupled to IRdye800, and with anti γ-tubulin (second row) and B23 (third row) monoclonal antibodies, detected with a secondary antibody coupled to Alexa680. (B) Nucleolin coimmunoprecipitates with ninein. Immunoprecipitation (IP) assay was performed in HeLa cells with anti ninein monoclonal antibody. Following immunoprecipitation, proteins were separated on a SDS-PAGE gel and probed with an anti nucleolin polyclonal antibody (polyclonal antibody 5567, lower row), detected with a secondary antibody coupled to IRdye800, and with anti ninein monoclonal antibody (upper row), detected with a secondary antibody coupled to Alexa680. IP without antibody (No Ab) and preimmune serum (IgG) were used as control. Input: input pre-immunoprecipitation fractions; IP: immunoprecipitated proteins; Supernatant: supernatant after immunoprecipitation.