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. 2015 Oct 22;5:15193. doi: 10.1038/srep15193

Figure 3. Increasing dTORC2 activity or directly promoting actin polymerization in young flies enhances LTM generated by a weak training protocol.

Figure 3

(A) Western blots reveal significantly increased dTORC2 activity (p-Akt Ser505) in brain extracts from young (3 d) WT flies treated with 10 μM A-443654. Actin is the loading control and the blot is representative of three independent replicates. (B) Normalization of p-Akt Ser505 levels (p-Akt Ser505 normalized to total Akt levels; n = 3 per group, unpaired t test, t = 4.815, **P < 0.01). (C) LTM is enhanced in young (3 d) WT flies treated with 10 μM A-443654 and given a weak training protocol (1 trial; n = 9 per group, unpaired t test, t = 2.758, *P < 0.05). (D) JPK enhanced LTM in young (3 d) WT flies given a single training trial (n = 7 per group, unpaired t test, t = 5.354, *P < 0.05). All data are presented as mean ± s.e.m.