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. 2015 Oct 22;5:15528. doi: 10.1038/srep15528

Figure 2. Representative profiles of ThT fluorescence as a function of fibrillisation time for reactions involving (A) and (E) wildtype recMoPrP; (B) and (F) recMoPrP-S169N; (C) and (G) recMoPrP-N173T; (D) and (H) recMoPrP-S169N/N173T.

Figure 2

Reactions depicted in panels (E–H) included the addition of preformed fibrils at time zero, whilst those in panels (A–D) were unseeded. Triplicate fibrillisation reactions were monitored for 24 hours with fluorescence readings taken every 5 minutes and plotted as points (Δ,ο,x) to which sigmoidal curves were fitted (solid lines).