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. 2015 Oct 22;5:15528. doi: 10.1038/srep15528

Figure 6. The unfolding transition of PrP variants in the presence of urea at pH 7 (♦) and pH 4 (■) at a concentration of 5 μM.

Figure 6

The ellipticity at 222 nm was monitored by circular dichroism, normalised, and the average of 3 readings were plotted against the increasing concentration of urea. A curve representing a bi-phasic transition was fitted to each set of data to allow the calculation of midpoints of the transitions (A) recMoPrP; (B) recMoPrP-S169N; (C) recMoPrP-N173T; (D) recMoPrP-S169N/N173T.