Table 1. Summary of results from this work.

Protein	Rigid loop?	Fibril formation	Oligomer formation	Thermal denaturation	Urea-induced denaturation	Molecular dynamics
recMoPrP	No	Rapid	Slow	Medium	Stable	Loop region can adopt β-turn or 3–10 helix; Tyr168 can be either solvent exposed or protected
recMoPrP-S169N	Yes	Slow	Rapid	Less stable	Less stable	Reduced overall stability; Loop region can adopt β-turn or 3–10 helix; Tyr168 can be either solvent exposed or protected
recMoPrP-N173T	No	Rapid	Slow	Medium	Stable	Loop region can adopt β-turn or 3–10 helix; Tyr168 only solvent protected
recMoPrP-S169N/N173T	Yes	Slow	Very rapid and large oligomers formed	More stable	Less stable	Loop region only adopts a β-turn; Tyr168 only solvent exposed