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. 2015 Apr 18;14(12):1830–1841. doi: 10.1080/15384101.2015.1033594

Figure 2.

Figure 2.

Regulation of nuclear receptors expression during transdifferentiation. (A) Absolute quantitative realtime PCR analysis of the expression of PPARs and RXRα in C2C12 cells. The C2C12 cells were cultured in 10% FBS DMEM medium, and harvested at 80% confluence for analysis. The copy number of genes was all normalized to that of β-actin. All values are represented as mean ± SD from 3 independent experiments. (B) Real-time PCR analysis of the expression patterns of nuclear receptors during transdifferentiation from cells treated as in (Fig. 1B). All values are represented as mean ± SD from 3 independent experiments. The variance analysis was performed between the same time point of “Control” and “EPA.” The significance is presented as *P < 0.05, **P < 0.01. (C) Real-time PCR analysis of the expression patterns of cyclin D1 and nuclear receptors during transdifferentiation from cells treated as in (Fig. 1B).