Figure 6.

Leptin stimulates IEC-6 cell proliferation in a mTOR dependent manner. Cells were incubated with leptin (20 nM) in the absence or presence of rapamycin (20nM). After incubations the cells were incubated with Crystal Violet. (A) Image of the plate wells of cells treated for 120 h. (B) Quantification of the Crystal Violet stain absorbance after extracting it from the culture plates in different time points. (*) represent significant differences between leptin treated and control; and (+) represent significant differences between leptin treated and leptin plus rapamycin groups; according to the Student's T test when p < 0.05.