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Leptin stimulates cell cycle entrance and progression of IEC cells in a mTOR dependent manner. Cells were incubated with leptin (20 nM) in the absence or presence of rapamycin (20nM) for 48 hours. After incubation, cell cycle progression was evaluated by propidium iodide staining and flow cytometry analysis. The percentages of cells in each stage of the cell cycle (G0/G1, S, and G2/M) are indicated.
