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. 2014 Oct 30;13(19):3089–3199. doi: 10.4161/15384101.2014.949491

Figure 4.

Figure 4.

dhc-1(dot168) substitution and cyb-3 amplification work together to increase fitness in the absence of MDF-1. (A) oaCGH plots of 190 kb duplication located on Chromosome V. The y axes represent the log2 ratio of signal intensity of the region in F170 (green) versus N2, F270 (purple) versus N2, and F470 (blue) versus N2. The X axes represent genomic position on Chromosome V. The reference line intersecting at the point 0 reflects wild-type copy number, 2x depicts duplication (F170), while 3x is indicative of triplication (F270 and F470). (B) qRT-PCR was used to confirm the copy number variations affecting the cyb-3 locus in our strains. (C) cyb-3 amplification and dhc-1 act together to increase fitness in the absence of MDF-1. Fitness was measured as the percent of progeny that develop into fertile hermaphrodites. Error bars represent SEM (n = 8 trials for unc-46 mdf-1 such-4 and unc-46 mdf-1 cyb-3trip strains; n = 5 trials for the dhc-1-containing strains).