Table 1. MSCs only mildly affect co-stimulatory molecule expression of LPS-stimulated DCs.
| DCs only | ½ MSC per DC | 1 MSC per DC | 2 MSCs per DC | |
|---|---|---|---|---|
| Percentages | ||||
| CD80 | 95.5 ± 0.3 | 97.5 ± 0.4 | 98.2 ± 0.1 | 98.2 ± 0.1 |
| CD86 | 68.1 ± 0.7 | 73.8 ± 0.3 (***) | 76.0 ± 0.1 (***) | 76.5 ± 0.1 (***) |
| CD40 | 88.9 ± 0.3 | 92.1 ± 2.0 | 90.1 ± 0.8 | 93.43 ± 0.2 |
| OX40L | 35.5 ± 0.8 | 47.6 ± 3.7 (*) | 43.2 ± 1.1 | 44.1 ± 0.4 |
| CD30L | 18.2 ± 1.9 | 17.6 ± 12 | 19.5 ± 1.2 | 21.1 ± 1.5 |
| PD-L2 | 50.9 ± 0.6 | 56.5 ± 2.3 | 61.8 ± 0.9 (**) | 62.5 ± 0.15 (**) |
| MFI | ||||
| CD80 | 12666 ± 118 | 15568 ± 407 (***) | 16351 ± 321 (***) | 16961 ± 272 (***) |
| CD86 | 49906 ± 446 | 55290 ± 957 (***) | 52075 ± 55 | 49102 ± 75 |
| CD40 | 12447 ± 177 | 12065 ± 1148 | 11825 ± 287 | 12622 ± 12 |
| OX40L | 7439 ± 78 | 7655 ± 221 | 8196 ± 139 | 8135 ± 91 |
| CD30L | 2943 ± 41 | 2953 ± 53 | 2924 ± 32 | 3018 ± 59 |
| PD-L2 | 5469 ± 105 | 5012 ± 100 | 4695 ± 211 | 4534 ± 76 (*) |
DCs were co-cultured with indicated ratios of MSCs for 3 hours prior to addition of 100 ng/mL LPS for 24 hours. DC numbers remained constant. Co-stimulatory molecules on DCs, determined as CD11c+MHCII+, were determined by flow cytometry. The percentage and mean fluorescence intensity (MFI) is shown. All values are expressed as mean ± SEM and representative of two independent experiments done in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001.