Figure 8. RT-PCR determination of LAMP1 and LAMP2 mRNA levels in mouse fibroblasts treated as indicated for 24 h.

(A) The ratio LAMP/GAPDH has been normalized to 1 in each control cells well of the 5 series of wells and the results are expressed as fold increase vs. controls. Kruskal–Wallis test indicated that both LAMP1 and LAMP2 values were heterogeneous (P < 0.01 for each set). ^∗ P < 0.05; ^∗∗ P < 0.01; ^∗∗∗ P < 0.001 vs. controls (Dunn’s multiple comparison test for selected pairs). (B, C) Quinacrine-induced TFEB nuclear translocation in mouse fibroblasts transiently expressing the myc-tagged protein (direct immunofluorescence in fixed and permeabilized cells). Sample photomicrographs are shown in (C). Histograms represent the proportion of cells with a definite nuclear labeling, indicating TFEB nuclear translocation. The numbers of evaluated cells (positive for any staining) in three separate experiments are indicated above bars. χ² statistics: ^∗ P < 0.01, ^∗∗ P < 0.001 vs. the control cells.