Figure 6.
For figure legend, see page 2718.Figure 6 (See previous page). Suppression of the expression and activation of transcription factor Slug and TGF-β signaling by CD151. (A) Expression of Snail and Slug proteins and TGF-β-mediated signaling in mammary tissues. Mammary tissues from 3 individual mice per genotype were blotted with the indicated antibodies. MMP-2 served as a loading control. (B) Representative images of Slug distribution in mammary glands. IHC staining was conducted with tissues from CD151 WT (a, c) or KO (b, d) mice (n = 3). Scale bar: 50 μm. (C) Changes in Slug expression in human mammary epithelial cells upon CD151 ablation. Human mammary epithelial cells (HCC-1187 and SUM-149) with (KD) or without CD151 knockdown (CT) were lysed and blotted for Slug, CD151, α3 integrin and β-actin. (D) Effect of CD151 removal on Slug protein stability. Top panel, Scatter plot of fold changes in Slug protein over time. MCF-10A cells with or without CD151 knockdown were treated with 20 μg/mL cycloheximide over the indicated periods of time and followed by lysing in RIPA buffer and blotting with indicated antibody. (E) Proliferation indices of human mammary progenitor-like epithelial cells (HCC-1187, SUM-149) with (KD) or without CD151 knockdown (CT) over 48 hr period of culture (n = 4). *: P value <0.05. (A–D): Fold changes were calculated on the basis of densitometry measurements.