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. 2015 Feb 25;14(7):950–958. doi: 10.1080/15384101.2015.1014147

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Figure 1. — KDM4D region spanning 350–474 amino acids binds PAR in vitro. PAR-binding assay with 6xHis tagged KDM4D full-length (FL) protein (523aa) (A), deletion mutants: N-terminal (1–350aa) (B), GST-tagged C-terminal (350–523aa) (C), truncated C-terminal (1–474aa) and internal deletion 350–474aa (D), and 6xHis tagged KDM4D-4M mutant (contains 4 mutations: E357A, R450A, R451A and R455A) (E). 6xHis-Rpn8, GST-only and BSA are used as negative controls and H3 as a positive control. Right: schematic representation of KDM4D mutants. IB: Immunoblot. ³²P: radiolabelled PAR.