Figure 1.

4 EGI-1 inhibits mTORC1 signaling independent from its inhibition of cap-dependent protein translation. (A and B) Breast cancer cell lines MCF7 and ZR-75–1 were treated with DMSO or 50μM 4EGI-1 and collected at various time points. Phosphorylation status of downstream mTORC1 effectors was analyzed using protein gel blot with indicated antibodies. (C) Both MCF7 and ZR-75–1 cells were treated with DMSO, 50 μM 4EGI-1 or 50 μM Ribavirin for various times. Cell lysates were analyzed by immunoblotting with indicated antibodies. (D) Control and EIF4E-depleted MCF7 and ZR-75–1 cells were grown in 5% FBS and collected to perform western blot analysis with indicated antibodies.