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. 2015 Jan 21;14(2):232–242. doi: 10.4161/15384101.2014.977096

Figure 2.

Figure 2.

4E-BP1 knockdown did not restore the inhibitory effect of 4EGI-1 on cell proliferation. MCF7 (A) and ZR-75–1 (B) cells were transfected with control siRNA or 2 different 4EBP1 siRNAs. After 72 h, the cells were collected and used for protein gel blot analysis. MCF7 (C) and ZR-75–1 (D) cells were transfected with control siRNA or 2 different 4EBP1 siRNAs. Twelve hours after transfection, cells were seeded into 96-well plates and cultured for an additional 24 h and then treated with DMSO or 4EGI for 48 h. After that, cell number was determined using the CCK8 assay and normalized to the corresponding control. The data presented are the mean from 3 independent experiments. N.S indicates that data between compared samples are not significant.