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. 2015 Jan 21;14(2):232–242. doi: 10.4161/15384101.2014.977096

Figure 3.

Figure 3.

4EGI-1 increases Akt phosphorylation independent from its inhibitory role on cap-dependent translation initiation. MCF7 (A) and ZR-75–1 (B) cells were treated with DMSO (-) or 50 μM 4EGI-1 (+) for the indicated times followed by preparation of whole cell protein lysates. The indicated proteins were detected using western blot analysis. Relative expression of pAKT(S473) and pAKT(T308) after the indicated times of treatment with 4EGI-1 was quantified from 3 independent experiments as shown in (a). Columns, mean percentage of 3 independent experiments; bars, SD. *P < 0.05 (C) MCF7 and ZR-75–1 cells were treated with DMSO (-), 50 μM 4EGI-1 (+) or 50 μM Ribavirin (+) for the given times and then harvested to prepare whole cell protein lysates followed by protein gel blot analysis using indicated antibodies. Control and EIF4E-depleted MCF7 and ZR-75–1cells (D) were grown in 5% FBS and harvested for the preparation of whole-cell protein lysates. Western blot analysis was performed with indicated antibodies.