Figure 5.

HoxB8 expression patterns. (A) Evaluation of HoxB8 gene expression by in situ hybridization. Black arrowheads highlight asymmetrical expression around the Henson's node. (B) Representation of the percentage of embryos that display HoxB8 expression. Numbers indicate the experimental N. Red boxes highlight the developmental stage where over 50% of the tested embryos present HoxB8 staining. (C) In situ hybridization images obtained with increasing times of staining, evidencing graded HoxB8 expression. (D) Transverse section analysis of HoxB8 expression patterns in different developmental stages. hf – head fold; hn – Hensen's node; ps – primitive streak; ect – ectoderm; end – endoderm; epi – epiblast; hyp – hypoblast; nt – neural tube; s – somite; psm – presomitic mesoderm; fg – foregut; nc – notochord.