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. 2014 Oct 30;13(18):2847–2852. doi: 10.4161/15384101.2014.949201

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© 2014 The Author(s). Published with license by Taylor & Francis Group, LLC

© Rasika Mundade, Hatice Gulcin Ozer, Han Wei, Lakshmi Prabhu, and Tao Lu

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 1. — Principle of ChIP assay. The protein-DNA complexes are crosslinked in the nucleus, so the protein of interest and its chromatin binding site can be fixed. After lysing the cells, the protein-DNA complexes are sonicated into 200-1000bp fragments, and immunoprecipitated by probing with specific antibody. The crosslinks of the protein-DNA complexes are then reversed, and DNA is further purified and subjected to further analysis.