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. 2015 Jan 21;14(7):1024–1035. doi: 10.1080/15384101.2015.1007015

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© 2015 The Author(s). Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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(See previous page). CEP161 regulates nuclear and centrosomal number and affects cell size and growth. (A) Nuclei number per cell. (B) Nucleus-centrosome distance. The percentage of cells with the indicated distances is given. (C) Nucleus-centrosome ratio. The percentage of cells is given. A total of 600 cells were counted for AX2 and mutant strains for each of the experiments in A, B, and C. (D) Confocal images for D1 and D2 cells showing increased centrosome numbers. DAPI was used to stain the nucleus. Scale bar, 5 μm. (E) Cell size of the strains in micrometers. (F) Growth on K. aerogenes. (G) Growth in axenic medium in shaking culture. (H) Phagocytosis was assayed using the strains from (G) and TRITC-labeled yeast. Approximately 200 cells from each strain were counted. The percentage of cells which had engulfed yeast particles after 15 min is shown in the graph (***P < 0.001).