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. 2015 Jan 21;14(7):1024–1035. doi: 10.1080/15384101.2015.1007015

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© 2015 The Author(s). Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 4. — CEP161 affects unicellular motility and multicellular migration. (A) Analysis of chemotactic cell motility of AX2 and mutants. Time-lapse image series were captured and stored on a computer hard drive at 30-second intervals. Images were taken at magnifications of 10X every 30 s. The DIAS software was used to trace individual cells along image series and calculate motility parameters. Speed refers to the speed of the cell's centroid movement along the total path; directionality indicates migration straightness; direction change refers to the number and frequency of turns; persistence is an estimation of movement in the direction of the path. Values are mean ± standard deviation of >30 cells from 3 or more independent experiments. The symbol * indicates the significance of the p-Value. *<0.5, **<0.01, ***<0.001. (B) Images showing the cell shape of aggregation competent cells of AX2, CEP161 and D3 cells. Scale bar, 50 μm. (C) Phototaxis assay. Slugs and slug trails were transferred to nitrocellulose filters and stained with amido black. The red arrow indicates the source of light. (D.) Angle of deviation of the slugs in response to light source. Slugs from four individual experiments were analyzed.